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KMID : 1156220180440030293
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Journal of Environmental Health Sciences
2018 Volume.44 No. 3 p.293 ~ p.300
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Antioxidant and Antimicrobial Activities of Trypsin-treated Pinus densiflora Ethanol Extract
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Moon Ki-Eun
Park Kyo-Hyun
Lee Beom-Zoo
Kim Bae-Hwan
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Abstract
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Objectives: We investigated the antioxidant and antibacterial activities of Pinus densiflora ethanol extracts (PDEE) treated with trypsine as a protease.
Methods: Various antioxidant activities were evaluated by measuring total contents of polyphenol and flavonoid, DPPH electron-donating ability and ABTS+ radical scavenging activity of test material. To compare the antibacterial activity, paper disc diffusion assay was performed against two resident bacteria in human skin (Staphylococcus aureus and Staphylococcus epidermidis).
Results: As for the total contents of polyphenol and flavonoid, and the electron-donating ability and ABTS+ radical scavenging activity, both PDEE and trypsin-treated Pinus densiflora ethanol extract (T-PDEE) showed high antioxidant activity in dose-dependent manner. And the T-PDEE showed slightly higher activity than PDEE, which indicated protease treatment seemed to affect in antioxidant activity. In the result of paper disc diffusion assay, antibacterial activity was confirmed in all two types of skin resident bacteria. T-PDEE was more active than PDEE and it seems that treatment of protease may increase the antibacterial activity of PDEE.
Conclusion: All of these results, we confirmed that treatment of protease to PDEE can increase the antioxidant and antibacterial activities, and it can be explained thought that this would be applicable as a cosmeceutical material in the future.
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KEYWORD
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Antibacterial activity, Antioxidant, Pinus densiflora, Protease, Trypsin
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